Elisa Protein Concentration Assay

Detergent Critical Micelle Concentration (CMC) Assay Kit

CMC1000 ProFoldin 1000 assays 187.8 EUR
Description: This product includes 100 ul of 1000 x CMC dye. It is for measurement of 1000 samples using 96-well plates. It can also be used for determination of detergent CMC values using cuvettes.

Assay Elisa Laboratories manufactures the elisa protein concentration assay reagents distributed by Genprice. The Elisa Protein Concentration Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Assay elisa. Other Elisa products are available in stock. Specificity: Elisa Category: Protein Group: Concentration Assay

McCown and Murashige and Skoog (MS) Vitamin Concentration (Syringe Vials)

Glycomatrix 100 mL 42.04 EUR

Gresshoff and Doy (DBM2) Vitamin Concentration (Syringe Vials)

Glycomatrix 100 mL 43.56 EUR

96 Well Plate RNA Cleanup and Concentration Kit

Bio Basic 2xPlates, 192prep 300.37 EUR

Goat True insulin ELISA kit

BlueGene 96T 700 EUR
Description: ELISA

Porcine True insulin ELISA kit

BlueGene 96T 700 EUR
Description: ELISA

Canine True insulin ELISA kit

BlueGene 96T 700 EUR
Description: ELISA

Mouse True insulin ELISA kit

BlueGene 96T 700 EUR
Description: ELISA

Concentration Assay information

Cas9 Nickase H840A NLS Protein (High Concentration)

K138 ABM 40 µg (250pmol) Volume: 25µL 135 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease. ;;To improve the off-target mutagenic effects of this system, the Cas9 Nickase H840A Protein was developed with a H840A mutation in its HNH-like nuclease domain. Unlike the Cas9 nuclease, this mutant form generates a single-stranded nick instead of a double-strand break (DSB). Because a single DNA nick is quickly repaired with high fidelity by the cellular machinery, the system requires two closely juxtaposed nicks in order to trigger the same genomic disruption as the Cas9 nuclease. This effectively boosts the recognition sequence to 40 instead of 20 nucleotides, and, as a result, off-target effects become highly unlikely. Thus, the double-nickase CRISPR system offers unparalleled specificity to satisfy even the most stringent of experimental requirements.;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First, the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 H840 Nickase NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First, the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 H840 Nickase NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

saCas9 Null Mutant NLS Protein (High Concentration)

K147 ABM 32.5 µg (250pmol) Volume: 25µL 155 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The saCas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type saCas9. Such a saCas9 protein retains its ability to bind to genomic DNA through gRNA:genomic DNA base pairing, however, the saCas9 Null Mutant does not introduce any genome modifications. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression.;; The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 NLS Null Mutant contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

Cas9 Null Mutant GFP NLS Protein (High Concentration)

K186 ABM 47µg (250pmol) Volume: 25µL 155 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;The Cas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type Cas9 (D10A and H840A). Such a Cas9 protein retains its ability to bind to genomic DNA through gRNA:genomic DNA base pairing, however, unlike Cas9 Nuclease and Cas9 Nickase, where permanent gene disruption can be achieved, the Cas9 Null Mutant does not introduce any genome modifications. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression. ;The Cas9 from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. ;The fusion of Cas9 to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Null Mutant GFP can also be used for FACS applications and screening. Cas9 D10A Null Mutant GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

Cas9 D10A Nickase GFP NLS Protein (High Concentration)

K149 ABM 47µg (250pmol) Volume: 25µL 155 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease. ;To counteract off-target mutagenic effects of this system, the Cas9 Nickase D10A was developed with a D10A mutation in its RuvC1 nuclease domain. Unlike the Cas9 nuclease, this mutant form generates a single stranded nick instead of a double-strand break (DSB). Because a single DNA nick is quickly repaired with high fidelity by the cellular machinery, the system requires two closely juxtaposed nicks in order to trigger the same genomic disruption as the Cas9 nuclease. This effectively boosts the recognition sequence to 40 instead of 20 nucleotides, and, as a result, off-target effects become highly unlikely. Thus, the double-nickase CRISPR system offers unparalleled specificity to satisfy even the most stringent of experimental requirements. ;The Cas9 from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. ;The fusion of Cas9 to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 D10A Nickase-GFP can also be used for FACS applications and screening. Cas9 D10A Nickase -GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

ETHANOL, 70% CONCENTRATION

IB15727 IBI Scientific 10L 210.13 EUR
Description: BOX W/SPIGOT (HAZ)

ddATP - high concentration

NU-271L Jena Bioscience GmbH 5 x 10µl 146.8 EUR

ddATP - high concentration

NU-271S Jena Bioscience GmbH 10µl 51.9 EUR

ddCTP - high concentration

NU-272L Jena Bioscience GmbH 5 x 10µl 146.8 EUR

ddCTP - high concentration

NU-272S Jena Bioscience GmbH 10µl 51.9 EUR

ddGTP - high concentration

NU-273L Jena Bioscience GmbH 5 x 10µl 146.8 EUR

ddGTP - high concentration

NU-273S Jena Bioscience GmbH 10µl 51.9 EUR

ddTTP - high concentration

NU-274L Jena Bioscience GmbH 5 x 10µl 146.8 EUR

ddTTP - high concentration

NU-274S Jena Bioscience GmbH 10µl 51.9 EUR

Myoglobin Concentration ELISA Quantitation Kit

GWB-E1E945 GenWay Biotech ELISA_Kits Ask for price

pSET152- Low concentration

PVT11339 Lifescience Market 2 ug 444 EUR

Non-Interfering Protein Concentration Determination Kit

SK3071 Bio Basic 250Assays, 250preps 130.99 EUR

pSET152- high concentration

PVT11338 Lifescience Market 2 ug 444 EUR